Stability Indicating Double Divisor Spectrophotometric Method for determination of Diloxanide Furoate and Metronidazole in Their Binary Mixture

Diloxanide furoate (DXF) and Metronidazole (MTZ) are commonly combined as a binary mixture for the treatment of a variety of diseases brought on by bacteria and parasites including amoebiasis and giardiasis. Different analytical methods were established for determination of both DXF and MTZ, However, only one chemometric spectrophotometric method was established for their stability indicating determination which involves problematical steps and specific software program. Herein, double divisor ratio spectra derivative (DDRSD) method is exploited for quantification of DXF and MTZ in presence of DXF-degradation products (FUR and DEG). The recommended DDRSD method is linear over the range of 2–25 and 1–25 µg/mL for DXF and MTZ, correspondingly. Additionally, the suggested method was effectively employed to their pharmaceutical formulations with good findings. Besides, the recommended DDRSD method was validated conforming to ICH guidelines. The introduced DDRSD method is simple, selective, and economic and could be implemented for quality control samples of the studied drugs.